The development of an efficient pilot-scale leghemoglobine production technology, based on recombinant Pichia pastoris and Kluyveromyces lactis fed-batch fermentations. (BioHeme)

Project status


Project execution time

01.01.2022 to 30.11.2023

Project type


Project number

Project manager

Juris Vanags

Involved laboratories

Laboratory of Bioengineering

Call, activity

Specific Objective 1.1.1 "Improve research and innovation capacity and the ability of Latvian research institutions to attract external funding, by investing in human capital and infrastructure" measure “Support for applied research”

Project goal

Project goal is to develop effective fed-batch fermentation and scale-up techniques for the production of plant-based meat substitutes, utilizing a recombinant LegH (leghemoglobine) producing strain of Pichia pastoris and Kluyveromyces lactis.

Main activities of the project:

The main activities of the project include the creation of two genetically modified yeast strains capable of utilizing cheap and freely available substrates for hemoprotein biosynthesis. As well as to develop the following: efficient fermentation technologies for both previously obtained yeast strains; mathematical models of processes; efficient model-based control systems that would allow automatic control of hemeprotein biosynthesis processes in both batch and fed-batch mode. In addition, the project plans to perform theoretical and practical scaling of hemeprotein production processes to a pilot scale, as well as to evaluate the commercialization potential of the developed fermentation process and related systems.

Project duration: 23 months.

Total eligible costs of the Project account for 540 540 EUR, including funding from the European Regional Development Fund in amount of 81.96 % – 443 026.58 EUR.